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KMID : 0368420040470030210
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Journal of Plant Biology
2004 Volume.47 No. 3 p.210 ~ p.215
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Characterization of a RAPD fragment unique to species with hairy fruit skin in the genusActinidia
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Kim Seong-Cheol
Jung Yong-Hwan
Kim Mi-Sun
Koh Seok-Chan
Song Kwan-Jeong
Kim Ho-Bang
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Abstract
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To develop a SCAR primer related to the hairy-fruit trait in the genusActinidia, we took a PCR-RAPD approach using arbitrary 10-mer primers. PCR with the UBC 376 primer generated specific fragments from three species with hairy fruit skin. Those fragments were then cloned to determine their nucleotide sequences. Two SCAR primers were designed from the UBC 376 primer and nucleotide sequences were obtained from the PCR fragments. A SCAR primer, OKC385, specifically amplified a 385-bp fragment from one clone ofActinidia eriantha, four ofActinidia chinensis, and four ofActinidia deliciosa. Deduced amino acid sequences of this fragment showed high sequence homology with plant cellulose synthases, which are involved in the biosynthesis of cellulose, a major cell wall component. The 385-bp fragment was specifically detected only in the seriesPerfectae C.F. Liang of sectionStellatae Li. This type has many hairs on the leaves, fruits, and stems, suggesting that the gene containing the PCR fragment is involved in hair formation in this phylogenetic group. Taken together, our results suggest that the SCAR primer, OKC385, can be used as a specific primer for early selection of the non-hair trait in breeding of the genusActinidia.
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KEYWORD
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Actinidia, cellulose synthase, RAPD, SCAR primer
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